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KMID : 0364819860240020127
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Korean Journal of Microbiology
1986 Volume.24 No. 2 p.127 ~ p.132
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Asymbiotic Nitrogen Fixation of R. japonicum in Soybean Nodule Extract
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金聖號/Kim, S.H
李渶/金晶鎭/兪薏東/閔泰薏/Rhee, Y/Kim, C.J/Yoo, I.D/Mheen, T.I
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Abstract
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1
Soybean nodule extract was prepared and tested for the effectiveness in the induction of asymbiotic nitrogen fixation of R. japonicum P-168. A Asymbiotic nitrogenase activity was increased over twice when glutamate was replaced by nodule extract in the induction media. Independently of the induction media, the nitrogenase activity in the assay media was also enhanced by the addition of nodule extract (100-400pg protein/mn. The amount of ethylene in the assay media reached the highest point after 8 days incubation of R-168 and was decreased thereafter. The growth of R. japonicum R-168 was sensitive to the concentration of nodule extract. As a while, the effect of soybean root extract was not detected both in the induction of nitrogenase activity and in the growth of R. japonicum R-168.
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KEYWORD
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